Study of Various Virulence Genes, Biofilm Formation and Extended-Spectrum β-lactamase Resistance in Klebsiella pneumoniae Isolated from Urinary Tract Infections

Table 1: Genes, sequences of the used primers and the bp of the used products.

Gene	Sequence	bp	Positive Control
FimH-1	F: 5^/-ATG AAC GCC TGG TCC TTT GC-^/3 R: 5^/-GCT GAA CGC CTA TCC CCT GC-^/3	688	E. coli 700336
mrkD	F: 5^/-CCA CCA ACT ATT CCC TCG AA -^/3 R: 5^/-ATG GAA CCC ACA TCG ACA TT-^/3	240	K. pneumoniae ATCC BAA-2473
magA (K1)	F: 5^/-GGT GCT CTT TAC ATC ATT GC-^/3 R: 5^/-GCA ATG GCC ATT TGC GTT AG-^/3	1282	Klebsiella pneumoniae subsp. pneumoniae Strain K6 ATCC 700603
wzy (K2)	F: 5^/-GAC CCG ATA TTC ATA CTT GAC AGA G-^/3 R: 5^/-CCT GAA GTA AAA TCG TAA ATA GAT GGC-^/3	641	Klebsiella pneumoniae subsp. pneumoniae Strain K6 [ATCC^® 700603™
rmpA	F: 5^/-ACT GGG CTA CCT CTG CTT CA -^/3 R: 5^/-CTT GCA TGA GCC ATC TTT CA-^/3	535	Klebsiella pneumoniae 1100770
uge	F: 5^/-TCT TCA CGC CTT CCT TCA CT-^/3 R: 5^/-GAT CAT CCG GTC TCC CTG TA-^/3	534	K. pneumoniae ATCC BAA2473
kfu	F: 5^/- GAA GTG ACG CTG TTT CTG GC -^/3 R: 5^/-TTT CGT GTG GCC AGT GAC TC-^/3	797	Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan ATCC13882
Aerobactin	F: 5^/-GCA TAG GCG GAT ACG AAC AT-^/3 R: 5^/- CAC AGG GCA ATT GCT TAC C T-^/3	556	Klebsiella pneumoniae subsp. pneumoniae Strain K6 [ATCC 700603
bla_TEM	F: 5^/-ATA AAA TTC TTG AAG ACG AAA -^/3 R: 5^/- GAC AGT TAC CAA TGC TTA ATC -^/3	1080	K. pneumoniae ATCC 7881
bla_SHV	F: 5^/- GGG TTA TTC TTA TTT GTC GC -^/3 R: 5^/-TTA GCG TTG CCA GTG CTC-^/3	930	K. pneumoniae ATCC 7881
bla_CTX-M	F: 5^/-GCT ATG TGC AGT ACC AGT AA-^/3 R: 5^/-ACC AGA ATG AGC GGC GC-^/3	585	K. pneumoniae ATCC 7881