| Bacterial Strain | Relevant Characteristics | Culture Medium Used in this Work | Purpose |
|---|---|---|---|
| Klebsiella variicola T29A | Isolated from sugar cane [48], highly tolerant to desiccation and promoter of plant growth [47] | MacConkey (casein peptone 1.5 g/L, gelatin peptone 17 g/L, meat peptone 1.5 g/L, lactose 10 g/L, bile salts 1.5 g/L, Sodium Chloride 5 g/L, neutral red 0.03 g/L, crystal violet 0.001 g/L, agar 13.5 g/L) | Selection medium |
| MESMA (glucose 2.7 g/L, mannitol 1.8 g/L, yeast extract 2.7 g/L, MES 4.4 g/L, KH2PO4 0.65 g/L, K2HPO4 4.8 g/L) [50] | Growth medium for desiccation assays | ||
| MM9-Citrate [51] | Medium used in random mutagenesis assays | ||
| Escherichia coli CC118 λpir | Strain (λpir +), donor of the pUT mini-Tn5Km plasmid. The plasmid only self-replicates in the presence of the λpir protein and is suicidal in other genetic backgrounds. | LB (casein peptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L) -kanamycin containing 80 μg/mL of antibiotic (LB-Km80) [52] | Medium used in random mutagenesis assays |
| Escherichia coli RK600 | Helper strain, contains plasmid RK600, tra + | LB-chloramphenicol (LB-Cm30) | The medium used in random mutagenesis assays |
| Klebsiella variicola T29A::mini-Tn5Km (from mutant 1 to 8974) | Mutants that acquired the mini-Tn5Km transposon in some region of its genome | MM9-Citrato-Km80 | The medium used for the selection of mutants that acquire the mini-Tn5Km transposon |