Bacterial Strain |
Relevant Characteristics |
Culture Medium Used in this Work |
Purpose |
Klebsiella variicola T29A |
Isolated from sugar cane [48], highly tolerant to desiccation and promoter of plant growth [47] |
MacConkey (casein peptone 1.5 g/L, gelatin peptone 17 g/L, meat peptone 1.5 g/L, lactose 10 g/L, bile salts 1.5 g/L, Sodium Chloride 5 g/L, neutral red 0.03 g/L, crystal violet 0.001 g/L, agar 13.5 g/L) |
Selection medium |
MESMA (glucose 2.7 g/L, mannitol 1.8 g/L, yeast extract 2.7 g/L, MES 4.4 g/L, KH2PO4 0.65 g/L, K2HPO4 4.8 g/L) [50] |
Growth medium for desiccation assays |
MM9-Citrate [51] |
Medium used in random mutagenesis assays |
Escherichia coli CC118 λpir |
Strain (λpir +), donor of the pUT mini-Tn5Km plasmid. The plasmid only self-replicates in the presence of the λpir protein and is suicidal in other genetic backgrounds. |
LB (casein peptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L) -kanamycin containing 80 μg/mL of antibiotic (LB-Km80) [52] |
Medium used in random mutagenesis assays |
Escherichia coli RK600 |
Helper strain, contains plasmid RK600, tra + |
LB-chloramphenicol (LB-Cm30) |
The medium used in random mutagenesis assays |
Klebsiella variicola T29A::mini-Tn5Km (from mutant 1 to 8974) |
Mutants that acquired the mini-Tn5Km transposon in some region of its genome |
MM9-Citrato-Km80
|
The medium used for the selection of mutants that acquire the mini-Tn5Km transposon |