| Primers | Sequence (5' → 3') | Annealling Temperature (°C) |
|---|---|---|
| mini-Tn5KmFw | TATGCCTCTTCCGACCATCA | 61 |
| mini-Tn5KmRv | CCGACTCGTCCAACATCAAT | |
| ARB1A | CCACGCGTCGACTAGTACNNNNNNGATAT | 40 |
| TNEXT2 | CTTTATTGATTCCATTTTTACACT | |
| CEKG2B | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC | |
| ARB2A | CCACGCGTCGACTAGTAC | 60 |
| TNINT | GACCTGCAGGCATGCAAGCTTCGGC | |
| Tn5-Rv | GGTACCGAGCTCGAATTCGG | 60 |
| Mut 6 stop-Fw | CAGTTTTCATGTTCGGTTTC | |
| Tn5-Fw | GACCTGCAGGCATGCAAGCTTCGGC | 60 |
| Mut 6 start-Rv | AGTCATGCTCCTTCAGGAAC | |
| Mut 6 start-Rv | ATGGTACCAGTCATGCTCCTTCAGGAAC | 64 |
| Mut 6 stop-Fw2 | CCGAGCTCATTCCAATTATAGAATCACC | |
| pJET1.2 forward sequencing primer | CGACTCACTATAGGGAGAGCGGC | 60 |
| pJET1.2 reverse sequencing primer | AAGAACATCGATTTTCCATGGCAG |