Table 2: Real-time PCR assays targeting the three IS elements and the internal control Phocine Herpes virus (PhHV).

PCR Assay	Label	Sequence (5’-3’)	References
IS481	Forward primer	CGG ATG AAC ACC CAT AAG CAT	[14]
	Reverse primer	CGA TCA ATT GCT GGA CCA TTT
	Probe	FAM-CCC GAT TGA CCT TCC TAC GTC GAC TC-BHQ1
IS1001	Forward primer	AAT TGC TGC AAG CCA ACC A	[6]
	Reverse primer	CCA GAG CCG TTT GAG TTC GT
	Probe	FAM-ACA TAG ACC GTC AGC AG-MGB
IS1002	Forward primer	CTA GGT CGA GCC CTT CTT GTT AAC	[6]
	Reverse primer	GCG GGC AAG CCA CTT GTA
	Probe	FAM-CAT CGT CCA GTT CTG TTG CAT CAC CC-BHQ1
PhHV	Forward primer	GGG CGA ATC ACA GAT TGA ATC	[15]
	Reverse primer	GCG GTT CCA AAC GTA CCA A
	Probe	FAM-TTT TTA TGT GTC CGC CAC CAT CTG GAT C-BHQ1