Table 2: Real-time PCR assays targeting the three IS elements and the internal control Phocine Herpes virus (PhHV).

PCR Assay Label Sequence (5’-3’) References
IS481 Forward primer CGG ATG AAC ACC CAT AAG CAT [14]
Reverse primer CGA TCA ATT GCT GGA CCA TTT
Probe FAM-CCC GAT TGA CCT TCC TAC GTC GAC TC-BHQ1
IS1001 Forward primer AAT TGC TGC AAG CCA ACC A [6]
Reverse primer CCA GAG CCG TTT GAG TTC GT
Probe FAM-ACA TAG ACC GTC AGC AG-MGB
IS1002 Forward primer CTA GGT CGA GCC CTT CTT GTT AAC [6]
Reverse primer GCG GGC AAG CCA CTT GTA
Probe FAM-CAT CGT CCA GTT CTG TTG CAT CAC CC-BHQ1
PhHV Forward primer GGG CGA ATC ACA GAT TGA ATC [15]
Reverse primer GCG GTT CCA AAC GTA CCA A
Probe FAM-TTT TTA TGT GTC CGC CAC CAT CTG GAT C-BHQ1

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