| Primer Name | Primer Sequence | Annealing Temperature (°C) |
Length of PCR Product (bp) |
| Kfu A | 5-GAAGTGACGCTGTTTCTGGC-3 5-TTTCGTGTGTGGCCAGTGACTC-3 |
58.7 | 799 |
| iucD | 5-TACAGACCGACCTCCGTGAA-3 5-CTGCGAGGGCTGACACATTA-3 |
58.7 | 203 |
| hmuR | 5-AAAAACCGCTATGGGCGAGA-3 5-GAAAGTGGAAAACCGCACCC-3 |
57.7 | 417 |
| iroN | 5- GAATGAAACTACCGCCCCCA-3 5-AGATACTCGCGGCATGTCAG-3 |
58.7 | 202 |
| yHPI | 5-GAAGTGACGCTGTTTCTGGC-3 5-TTTCGTGTGTGGCCAGTGACTC-3 |
57.7 | 620 |