Fig. (1) Map of agpP-opt expression construct. Optimized agpP gene sequence with added C-terminal His-tag was inserted downstream of the secretion signal and AOX1 promoter. AOX1 – P. pastoris AOX1 promoter; Signal peptide – secretion signal peptide of α-amylase, inulinase, glucoamylase or lysozyme genes; agpP-opt – optimized sequence of phytase gene from Pantoea sp.3.5.1; His-tag – C-terminal 6 histidine tag; EcoRI, MlyI, KpnI – restriction sites; pPINK-HC or pPINK-LC – yeast expression vectors.