Fig. (2) Immunoblot analysis of proteins in culture liquid of P. pastoris transformants. After 36 hours of methanol induction, western blot revealed the presence of a specific band with an apparent molecular mass of nearly 80 kDa in the culture medium of all tested transformants. A – pPINK-amylase-agpP transformants; B – pPINK-inulinase-agpP transformants; C – pPINK-glucoamylase-agpP transformants; D – pPINK-lysozyme-agpP transformants. Lanes 1-2 – genetic constructs based on pPINK-HC vector; lanes 3-4 – genetic constructs based on pPINK-LC vector; NT – non-transformed yeast.