Fig. (3) Extracellular phytase activity in P. pastoris transformants. Phytase activity in P. pastoris strain with an integrated construct based on pPINK-HC vector and Kluyveromyces maxianus inulinase gene signal sequence was the highest. Phytase activity was barely detectable in the intracellular lysates of all transformants (less than 0.2). Error bars on the graph indicate standard deviations (N=3).