Fig. (4) Time course of extracellular phytase activity in P. pastoris strain pPINK-HC-inulinase-agpP after induction (A) and dissolved oxygen (DO) concentration, biomass and phytase production by recombinant P. pastoris strain pPINK-HC-inulinase-agpP in the 5-liter bioreactor (B). Optimal time to harvest AgpP-P phytase was 36 hours post-induction. Error bars on the graph indicate standard deviations (N=3).