Fig. (5) SDS-PAGE of the AgpP-P phytase purification (A) and AgpP-P preparations before and after N-deglycosylation by Endo H (B). A: M – molecular mass marker; lane 1 – culture liquid of P.pastoris; lanes 2-6 – eluate from Ni-NTA column; lane 7 – wash fraction. B: M – molecular mass marker; lane 1 – AgpP-P before N-deglycosylation; lane 2 – AgpP-P after N-deglycosylation; lane 3 – Endo H. AgpP-P after deglycosylation by Endo H showed a single band with smaller molecular size, verifying that AgpP-P is indeed N-glycosylated