Table 1: One-step purification of AgpP-P phytase produced and secreted from P. pastoris by affinity chromatography on the Ni-Sepharose column.

Purification step	Total protein (mg)	Total phytase activity (U)	Specific activity (U/mg)	Purification (fold)	Yield (%)
Culture liquid	656	174	0.26	1	100
Affinity chromatography	31	107	3.45	13.3	61.5