Table 2: Restriction enzymes used for restriction digestion.

Restriction enzyme	Target	Cutting site	Cutting positions	Fragment sizes (bp)
SmaI	PCR amplified ureD gene of K. pneumoniae	CCC↓GGG	453/454	453, 372
PciI	PCR amplified magA gene of K. pneumoniae	A↓CATGT	958/962	958, 269