Fig. (1) Electrophoresis of PCR products on 1% agarose gel. A) IgA1 protease gene was amplified using Taq DNA Polymerase enzyme. Lane 1: DNA marker (1 Kbp), lane 2: PCR product; IgA1 protease (705 bp). B). Electrophoresis of the recombinant plasmid digested with XhoI and NdeI restriction enzymes. Lane 1: 1 Kbp DNA marker, 2: PCR product; vector without IgA protease (705 bp). C. Electrophoresis of recombinant IgA1 protease. M: protein marker, T0: BL21 bacteria containing recombinant pET28a without IPTG. T4: BL21 bacteria containing recombinant pET28a with IPTG (after 4 hours of incubation). EB1-EB2: Expressed recombinant protein.