Fig. (1) The result of multiplex PCR and single PCR assay for detection of Brucella genus: Lane M: Marker DNA 100 bp, Lane 1: PCR product for B4/B5 and F4/R2, Lane 2: PCR product for B4/B5, Lane 3: PCR product for F4/R2, Lane 4: PCR product for B4/B5 (Positive control), Lane 5: Negative control.