| References | Annealing Temperature | Amplicon size (bp) | Sequence 5'→3' | Primer | Target Gene Description |
| [9] | 60 | 171 | F: 5- AAGTGCGTCAACGAGCAGCT-3 R: 5- TTAGTGCTTTGTGACCATCGA -3 |
β-giardin -F β-giardin -R |
Giardia lamblia |
| [10] | 56 | 170 | F: 5- AAGCATTGTTTCTAGATCTGAG-3 R: 5- AAGAGGTCTAACCGAAATTAG-3 |
EH -F EH -R |
E. histolytica species |