Fig. (3) Identification of integration site. Genomic DNA from DinoHI (lane 1) and D. nodosus strain H1215 (lane 2) was digested with NruI (panel A) or NruI and HindIII (panel B) and probed in panel A with a 1.0 kb NruI fragment from DinoHI (probe A, Fig.1A) or in panel B with a 2.2 kb NruI-HindIII from DinoHI (fragmentII, Fig. 2A). Sizes in kb of molecular weight markers are shown on the left hand side.