The Catalytic Product of Pentachlorophenol 4-Monooxygenase is Tetra-chlorohydroquinone rather than Tetrachlorobenzoquinone

Fig. (7) Peroxidase activity assay of the S. chlorophenolicum strain ATCC 39723 cell culture and its cell lysate supernatant. (A). 1.5 mL bacterial cell culture + 1.5 mL reaction mixture containing 1% pyrocatechol; (B). 1.5 mL bacterial cell culture + 1.5 mL reaction mixture containing 1% pyrocatechol and 1.5% H₂O₂; (C). 300 µL of 50 mM phosphate buffer, pH 7.0 + 1.5 mL reaction mixture containing 1% pyrocatechol and 1.5% H₂O₂; (D). 300 µL of cell lysate supernatant + 1.5 mL reaction mixture containing 1% pyrocatechol and 1.5% H₂O₂.; and (E). 300 µL of boiled cell lysate supernatant + 1.5 mL reaction mixture containing 1% pyrocatechol and 1.5% H₂O₂.