Fig. (5). GST-Pmp23 purification and zymogram. A. SDS-12.5% PAGE gel stained with Coomassie blue. Lane 1, standard molecular mass markers; lane 2, purified GST-Pmp23.B. Zymogram analysis: SDS-12.5% PAGE gel containing Micro-coccus lysodeikticus cells. Lane 1, renaturation of purified GST-Pmp23; lane 2, purified GST-Pmp23 stained with Coomassie blue; and lane 3, standard molecular mass markers