Table 1: E. coli Strains

Strain Description Reference or derivation
MG1655 F! ilvG rfb-50 rph-1 λ! [29]
BL21(DE3) hsdS gal (λ cI857 ind-1 Sam7 nin-5 lacUV5-T7 gene 1) [30]
DH5αZ1 (Ф80d lacZΔM15) endA1 recA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 (λ att lacIq tetR Spr) [31]
CAG12028 MG1655 zcj-233::Tn10 [32]
TL524 MG1655 Δ(lacZYA-argF)U169 [33]
TST3 MC4100 malT::Tn10 [34]
BW25113 lacIq rrnBT14 ΔlacZWJ16 hsdR514
ΔaraBADAH33 ΔrhaBADLD78
[35]
AL1 BW25113 ΔglpE::Kmr FRT (pKD46) This work
HC1.1 BW25113 ΔpspE::Kmr FRT (pKD46) This work
HC6.1 TL524 ΔpspE::Kmr FRT P1(HC1.1 → TL524)
HC7.1 TL524 ΔpspE::FRT a FLP removal of Kmr from HC6.1
HC8.1 TL524 ΔpspE::FRT ΔglpE::KmrFRT P1(AL1 → HC7.1)
JLD17201 TL524 ΔglpE:: KmrFRT P1(AL1 → TL524)
JLD17204 TL524 ΔglpE::FRT a FLP removal of Kmr from JLD17201
FA035 TL524 ΔpspE::FRT ΔglpE::FRTa FLP removal of Kmr from HC8.1

a A 113-bp FRT “scar” remains following removal of the Kmr cassette by FLP recombinase [35].