Table 2: Comparison of Rhodanese Activities in Wild-Type and Sul-furtransferase-Deficient Strains

Strain Genotype Sp. act. (U/mg)a
TL524 Wild type 0.065
JLD17204 ΔglpE::FRT 0.066
HC7.1 ΔpspE::FRT 0.013
HC8.1 ΔpspE::FRT 
ΔglpE:: KmrFRT
0.003

a Cultures were grown in minimal glucose medium at 37°C and harvested at an OD600 of ~1.0. Specific activities were determined using crude extracts derived from sonicated cells and are expressed as units per mg protein. Substrate concentrations employed were 50 mM thiosulfate and 50 mM cyanide.