Fig. (5) Assessment of SeM-specific mucosal IgA and systemic IgG production in mice with the intranasal S. equi infection by ELISA and Western immunoblot. Nasal wash and serum samples were collected from the surviving mice in the nasal infections with wild-type and ΔvicK strains of Fig. 4B on day 30 after inoculation. A) immunoreactivity of SeM38-260 with IgA in the nasal wash samples. B) the reciprocal of anti-SeM38-260 IgG titers in the serum samples. C) immunoblots demonstrating anti-SeM38-260 IgG in the serum samples, which were diluted by 1:1000 in the assay.