| Strain and Plasmid | Deskription | Reference |
|---|---|---|
| E. coli strains | ||
| i484 | O25:H autoagglutinating, human isolate | 17 |
| oy077 | i484, fitR :: EZ-TN5< Kan-2 > | This study |
| oy016 | i484, lacZ :: EZ-TN5< DHFR-1 > | This study |
| oy021 | oy016 carrying pMP-fitB | This study |
| oy022 | oy016 carrying pMP-fitA | This study |
| oy026 | oy016, fitR :: EZ-TN5< Kan-2> | This study |
| oy028 | oy026 carrying pMP-fitB | This study |
| oy029 | oy026 carrying pMP-fitA | This study |
| oy071 | i484, fur :: EZ-TN5 <DHFR> | This study |
| oy078 | oy077, fur :: EZ-TN5<DHFR> | This study |
| DH5α | F–, ф80d lacZ∆M15 ∆ (lacZYA–argF) U169, endA1, recA1, hsdS17 (rK–mK+), deoR, thi–1, supE44, λ–, gyrA96, relA1 | Invitrogen |
| Plasmids | ||
| pMP220 | Contains a promoterless lacZ gene, Tetr | 19 |
| pMP–fitA | fit promoter was cloned into pMP220 with a direction of fitA transcription | 2 |
| pMP–fitB | fit promoter was cloned into pMP220 with a direction of fitB transcription | 2 |
| pBAD22 | overexpression vector, Ampr | 8 |
| pBAD–fitR1 | DNA fitR1 cloned into pBAD22 at EcoR I and Hind III sites | This study |
| pBAD–fitR2 | DNAfitR2 cloned into pBAD22 at EcoR I and Hind III sites | This study |
| pCR | Cloning vector pCR-XL-TOPO | invitrogen |
| pCR64 | DNA fitR1 cloned into pCR-XL-TOPO | This study |