Table 2.: Oligonucleotide Primers Used in this Study

  Gene Primer, 5’-3’
PCR and cloning    
  fitR1 forward CGGAATTCGCGAGTCAGTTAACGCCT
    reverse TATAAGCTTGCCATTCTTTCTTCCCGT
  fitR2 forward CGGAATTCATGATCCAGAAAGATAAAGTGCG
    Reverse TATAAGCTTTTAGTGATGGTGATGGTGATGGCCATTCTTTCTTCC
  fit promoter forward GTGCCACGTGTACGCCAACTTAAT
    reverse TCGCGGATTGTCGCGAGTGAAA
       
qPCR      
  fitA forward ACCATGCTCTATTCCCATCG
  reverse GGCTGTTTCGTCGTAAGGTC
  fitB forward GCACCATTGCACGTATCTTG
    reverse ATAACGCTTTCGTTGGTTGC
  fitC forward ACCTTGCACCCTCCCTTAAC
    reverse CAAGCAGGGTAAATGTTCGC
  fitD forward TCTGCTTGGTCATCAGGTTG
    reverse AACAACGCCAGACGAGAACT
  fitE forward TATCGGTGATTCAGGCAAACCAGG
    reverse GTAATACCCGTCCCAGCGAATGAT
  fitR forward ATGAGCATCGTGAAGTGGTACTCG
    reverse ATAGCCCTGATAACTGTGGCATCG
  fepA forward GCCGATTGATTTCCTCGTAA
    reverse ATCCGTTGCTGATTAAACTA
  gst forward GAAGCTGCAATATGTGAACGAGGC
    reverse AGCGCAGAACCGTAAACAGATAGG
  polA forward TGAGTTCAAACGCTGGACTG
    reverse TCTGCAACACTGGTTTCCTG

Restriction enzyme sites were depicted as underlined. In the reverse primer for fitR2, the 6 x his tag sequence was indicated in bold.