Fig. (3) Effect of IFN-b neutralization on B7-H1 and B7-DC expression.iDC were stimulated with flagellin, LPS, poly (I:C) and R848 for 48 h in the presence or absence of anti-IFN-b antibody (7.5 mg/ml).Panel A. IFN-b in the supernatant was quantified by ELISA. Data are shown as means ± standard deviations (error bars) of five experiments.*Samples that are significantly different (p < 0.05) from the control culture (DC alone). The pre-treatment of cell culture with saturing amount of anti-IFN-b antibody completely neutralized IFN-b in the supernatant.Panel B. Representative histograms showing the Mean fluorescence intensity of iDC stimulated flagellin, LPS, poly (I:C) or R848 alone, in the presence of anti-IFN-b mAb, or in the presence of the isotype-matched control mAb, stained with the indicted mAb. Of note, pre-treatment with saturing amount of anti-IFN-b antibody failed to suppress the TLR-induced upregulation of B7-H1 and B7-DC on iDC. Data are shown as means ± standard deviations (error bars) of five experiments.