Fig. (3) Evaluation of single-tube seminested and two-tube nested PCRs on HPV plasmids of genotypes 6,11,16,18,31,33,38 and 51. A: Single tube seminested PCR products with amplicons of 450 bp and/or 190 bp. B: Two-tube nested PCR wıth amplicons of 140bp after second round PCR. Non-specific bands are also visible.