Fig. (1) Rhamnolipid production by P. aeruginosa PAO1. Time-course of production of the three major rhamnolipid species (103 Area/OD600): di-rhamnolipids Rha-Rha-C10-C10 (ion m/z 649, squares), Rha-Rha-C10-C12 (ion m/z 677, circles), and Rha-Rha-C10-C12:1 (ion m/z 675, triangles) in PPGAS medium. The results are representative of at least three independent experiments.