Effects of Osmotic Stress on Rhamnolipid Synthesis and Time-Course Production of Cell-To-Cell Signal Molecules by Pseudomonas aeruginosa

Table 2: Highest Extra-Cellular Concentrations of HSLs and Quinolones in P. aeruginosa PAO1 Culture Supernatants

Medium	3OC₁₂-HSL (nM)^‡	C₄-HSL (nM)^‡	HHQ (nM)^‡	PQS (nM)^‡
PPGAS	160 ± 10	2140 ± 230	893± 92	2054± 176
PPGAS/NaCl^*	50 ± 4	410 ± 50	1525± 19	832± 67
PPGAS/NaCl/GB^†	190 ± 12	860 ± 95	1002± 95	2162± 210

^* 0.5 M NaCl was included in the PPGAS medium prior to bacterial inoculation.

^† 0.5 M NaCl and 1 mM GB were included in the medium.

^‡ The values are averages of at least two experiments and the standard deviations are given.