Effects of Osmotic Stress on Rhamnolipid Synthesis and Time-Course Production of Cell-To-Cell Signal Molecules by Pseudomonas aeruginosa

Table 3: Highest Extra-Cellular Concentrations of Rhamnolipids in P. aeruginosa PAO1 Culture Supernatants

Medium	Rha-Rha-C₁₀-C₁₀ (10³ Area/OD₆₀₀) ^‡	Rha-C₁₀-C₁₀ (10³ Area/OD₆₀₀) ^‡
PPGAS	50 ± 7.8	1.83 ± 0.12
PPGAS/NaCl^*	0	0
PPGAS/NaCl/GB^†	20 ± 1.6	0.92 ± 0.1

^* 0.5 M NaCl was included in the PPGAS medium prior to bacterial inoculation.

^† 0.5 M NaCl and 1 mM GB were included in the medium.

^‡ The liquid chromatography-mass spectrometry peak surface areas were divided by the OD₆₀₀ values of the cultures. The values are averages of at least two experiments and the standard deviations are given.