Fig. (4) Co-expression of either PLB1 or PLB2 or PLB3 with PLA2, respectively, in the plb123 triple mutant. All strains are designated by those plasmids expressed in the plb strain as designated in Table 1 (e.g. plbPLA2PLB1 for the plb triple mutant co-expressing the Arabidopsis PLA2 and the yeast PLB1). Transformants were cultured to the stationary phase in SC medium with glucose and adjusted to an OD600 of 3.0 and also diluted by 10, 100 and 1000-fold; 10 µl of each was spotted on plates with different media. The photograph was taken after 3 days of growth.