Fig. (2) Restriction analysis and PCR amplification using pAL79 DNA containing an insertion as a template.(A)Dra I digestion of recloned pAL79. Recloned DNA samples were prepared from 4 independent colonies, and both altered and control plasmids were examined. Lanes 1, 2, 4 and 5, Dra I-digested recloned altered pAL79; lane 3, Dra I-digested control pAL79; lane M, 100-bp DNA ladder size markers. The large blobs at the bottom are RNA that remained in the plasmid preparation. (B) Electrophoretic mobility of PCR-amplified fragments. Lane 1, normal control pAL79 used as a template; lane 2, altered pAL79 used as a template; lane M, 100-bp DNA ladder size markers.