Fig. (2) Heavy-metal sensitivity tests of the constructed yeast transformants. Three transformants, pYES2 transformant (control), bxa1 transformant and Δ35bxa1 transformant, were grown in YNB. Ag sensitivity test (A), Cu sensitivity test (B), Cd sensitivity test (C) and Zn sensitivity test (D). Log-phase cells of these strains were adjusted to OD₆₀₀ 1.0, serially diluted (1/20, 1/100, 1/500, 1/2500) and then spotted on YNB agar plates containing various concentrations of heavy metals. Plates were incubated at 30°C for 4 d.