Fig. (3) Heavy metal sensitivity tests of the constructed E. coli transformants. Three transformants, pBAD/ Myc-HisB transformant (control), bxa1 transformant and Δ35bxa1 transformant, were grown in YNB + L-arabinose. Ag sensitivity test (A), Cd sensitivity test (B) and Zn sensitivity test (C). Log-phase cells of these strains were adjusted to OD600 1.0, serially diluted (1/20, 1/100, 1/500, 1/2500) and then spotted on YNB agar plates containing various concentrations of heavy metals. Plates were incubated at 37°C for 2 d.