Fig. (7) (A) The secondary structures of the 5’ UTR region of the mRNA from (a) clpQ⁺ with ΔG = -24.6 Kcal/mole (b) ΔIR-clpQ⁺ with ΔG= -3.3 Kcal/mole and (c) ΔL-clpQ⁺ with ΔG = -1.3 Kcal/mole. (B) The βGal of cpsB::lacZ in the presence of pBAD33, pBAD33-clpQ⁺, pBAD33-ΔIR-clpQ⁺, or pBAD33-ΔL-clpQ⁺, while ClpY was induced. (C) The western blot of ClpQ, expressed, in series, from the above plasmids. The loading was normalized by the readings of OD₆₀₀. (D) The mRNA of clpQ⁺, which individually expressed from the above plasmids. The growth condition was as indicated earlier for the addition of antibiotics and 0.5 % arabinose, and the loadings were normalized by 16S rRNA (data not shown). (E) The decay of mRNAs of clpQ⁺ and ΔIR-clpQ⁺; the remaining amount of mRNA at different time points, after an addition of rifampin (at a final concentration, 200 µg/ml).