Table 2: P-values Calculated by the UniFrac Significance Test from Comparisons of the Bacterial Community Found in Stool Samples Immediately (IM), after 12h, 24h 48h and 72h Prior to DNA Extraction and Amplification of the16S rRNA

Subject A Subject B Subject C Subject D
Qualitative Quantitative Qualitative Quantitative Qualitative Quantitative Qualitative Quantitative
IM x 12h 1.00 1.00 1.00 0.74 1.00 0.98 0.93 1.00
IM x 24h 1.00 1.00 0.95 0.26 1.00 0.95 0.81 0.92
IM x 48h 0.59 0.93 0.32 0.09 0.73 0.97 0.23 0.63
IM x 72h 0.94 0.98 1.00 0.04 0.98 0.98 0.35 0.82

The comparison was made considering the presence/absence (qualitative) and the abundance (quantitative) of taxonomic unities. All P-values have been corrected for multiple comparisons by multiplying the calculated P-value by the number of comparisons made (Bonferroni correction). For each pairwise comparison, if the p-value is less than or equal to 0.001 the result indicates a highly significant difference in the composition of the communities sampled by each library. P-values from 0.001 to 0.01 indicate a significant difference, from 0.01 to 0.05 indicate a marginally significant difference, from 0.05 to 0.1 indicate a suggestive difference and if the p-values are greater than 0.1 the result indicate that the difference is not significant.