a)Murine macrophages were infected with MTB at MOI 1:1 for 2 days or at MOI 1:0.1 for 8 days and cultured in the presence of LPS or the MALP-2 derivative at the indicated concentration. Culture supernatants harvested at the indicated time points were used for IL-6, IL-10, TNF-α , MIP-1β detection by specific quantitative sandwich ELISA Kits. The results are presented as mean.
± SEM in pg/ml. Pooled data of 3 independent experiments are shown. Nitrite was detected at 2 days with a specific colorimetric kit. The results are presented as mean ± SEM in µ Mol/L. Pooled data of 3 independent experiments are shown.
* p<0.05 and
** p<0.01, level of statistical significance between cytokine or nitrite release in stimulated versus untreated macrophages.
# p<0.05 and
## p<0.01, level of statistical significance between cytokine or nitrite release in LPS-treated versus MALP-2 derivative-stimulated macrophages.