Fig. (1) Representative 2-DE gels of L. interrogans serovar Pomona protein samples. Whole-cell protein extracts of virulent Leptospira cultured from kidney (A) and liver (B) were separated by 2-DE, with pH 3-10 and molecular mass of 10-70 kDa. Protein spots were excised for identification by MALDI-TOF MS. Examples of known leptospiral proteins, including some characterized lipoproteins (**) and some novel hypothetical proteins (*) identified in this work are depicted.