Fig. (2) Activity of granulysin peptide against Y. pestis is growth temperature-dependent. CFU reduction of Y. pestis (CO 92) across granulysin peptide concentration when grown at 26°C and 37°C. Reduction of CFU following 3 h incubation with peptide was determined by overnight growth on HIB agar plates. Percentage reduction from control growth at representative peptide concentrations is shown in parenthesis. Data shown are mean ± SEM of five independent experiments. Compared to negative control, growth was significantly (p<0.01) reduced by 5 to 100 µM concentration of granulysin peptide at both 26 and 37°C. Effects of peptide on growth reduction were significantly different (p<0.05) at 26°C and 37°C from 5 to 100 µM concentration.