Table 1.: Species Distribution of 96 Nocardia Isolates by Sequence-Based Alignment of 606-bp 16S rDNA Fragments with Reference Sequences and BLASTn Against Database Sequences

Species identification (Alignment with Reference Sequences) No. Isolates/No. Matched by Phenotypic Identification No. Species with ≥99% Sequence Match (BLASTn) GenBank Range % Similarity/No. Matched Sequences1 BIBI Range % Similarity/No. Matched Sequences1 RDP-II Range % Similarity/No. Matched Sequences1
N. asteroides sensu stricto 2/2 1 99.0-100/3 100/3 100/2
N. abscessus 1/0 32 100/15 100/20 100/13
N. aobensis 1/0 1 99.8/4 99.8/4 98.8/4
N. arthritidis 1/0 13 99.6/13 99.6/13 0
N. beijingensis 2/0 1 100/13 100/18 98.1/13
N. brasiliensis 3/3 1 100/2 100/4 100/2
N. cyriacigeorgica 24/0 1 99.8-100/35 99.8-100/21 99.1-100/15
N. farcinica 13/10 24 99.8-100/14 100/12 100/8
N. nova 31/28 1 99.3-100/5 99.6-100/5 99.0-100/4
N. otitidiscaviarum 5/4 1 100/5 100/5 100/4
N. paucivorans 7/1 1 100/1 100/1 100/1
N. transvalensis 1/0 1 99.7/3 99.6/5 98.8/2
N. veterana 4/0 1 100/5 100/8 100/4
N. vinacea 1/0 1 100/3 100/3 100/2

1 Refers to the number strains in the database with a sequence similarity of ≥ 99% to that of the query sequence.
2 Refers to 100% sequence similarity with N. asteroides, N. asiatica and N. abscessus sequences.
3 99.6% sequence similarity to a single N. bejingensis sequence (GenBank accession no. AY756543) but <99% sequence similarity to other N. bejingensis strains. Since the sequence demonstrated 99.0% similarity to the reference N. arthriditis sequence, it was assigned as such.
4 Refers to 100% sequence similarity with N. farcinica sequences as well as with sequence of N. otitidiscavarium strain DSM 43242T (GenBank accession no. X80611).