Fig. (5) GSL binding of deacylGg₄. GSLs were applied to tlc plates dried and detected by orcinol spray for carbohydrate (upper panels). A separate series of GSL dot blots was incubated with deacylGg₄ and any bound Gg₄ detected by immunostaining with Mab antiGg₄ (middle panels). a) GM3 ganglioside, b) GM1 ganglioside, c) Gg3, d) Gb₄, e) LacCer. No reaction was seen in the absence of antiGg₄ or without incubation with deacylGg4. Bound deacylGg₄ detected for each GSL was quantitated using Image J (lower panel). DeacylGg₄ bound LacCer>Gg3>GM1.