Fig. (6) TLCK prevents P. aeruginosa internalization. A) S9 cells were pretreated with 50µM TLCK for 1hr at 37^°C prior to assay of P. aeruginosa internalization. The effect was compared to that of P4 GSL inhibition. B) the effect of TLCK on P. aeruginosa colonies for S9 cells with (internalized bacteria only) and without (internalized+host cell attached bacteria) gentamycin was measured. C) S9 cells (lane 1) were pretreated with P4 (lane 2) or 100µM AEBSF for 30mins. D) S9 cells (lane 1) were pretreated with P4 (lane 2), 10µM PPM-18 for 1hr (lane 3) or 50µM suramin overnight (lane 4). Data represent means ± SD of three experiments. * indicates p<0.05.