Fig. (3) Recombinant SeMac and evidence for in vivo production of SeMac. (A) SDS-PAGE analysis of recombinant SeMac. Lanes: 1, E. coli lysate without SeMac (control); 2, E. coli lysate containing overexpressed SeMac; 3, purified SeMac. (B) Western immunoblot analysis of SeMac with SeMac antibody present in the convalescent-phase sera from 3 horses with S. equi infection (eC1, eC2, eC3) and mice before (mP) and after (mC) experimental S. equi infection.