Table 1: The Amino Acid Sequences of the Lower Hinge Region of Human and Horse IgG Subgroups and Their Cleavability by GAS M1 Mac and SeMac

IgG Subgroup AA Sequence in the Lower Hinge Regiona Cleaved byb
M1 Mac SeMac
Human IgG1 HTCPPCPAPELLGGPSV + +
Human IgG2 VE.......PVA-.... + +
Human IgG3 PP..R............ + +
Human IgG4 PP..S.....F...... + +
Horse IgG1 SK..KC........... + +
Horse IgG2 PQ...YTHSKF...... ND ND
Horse IgG3 CE..K............ ND ND
Horse IgG4 GGC.T..PEC.SV.... - -
Horse IgG5 SP..K......P..... ND ND
Horse IgG6 VIKE..CC.KCP.R... ND ND
Horse IgG7 CGGC.TC..C.SV.... ND ND

a Cleavage was prior to the Gly residue underlined and determined by N-terminal amino acid sequencing of the proteolytic products in the reaction catalyzed by GAS M1, Mac and dots indicate identical residues with human IgG1.
b+ cleaved;
- not cleaved;
ND not determined.