Table 1: General Information about the Number of Clones and Ribotyes, Estimated Percentage of Ribotypes Captured and Bacteria Diversity in Formosan Subterranean Termite (FST) Samples from Louisiana and China. For Comparison, the Same Indices were Calculated Based on Previously Published Data for one FST Colony from Japan [14]

LA Fresh LA EtOH China EtOH Japan
FST samples 1 2 3 4 5 6 7 8 9 10
General information
Clones sequenced 350 248 276 123 161 177 172 238 131 250
Ribotype richness 57 51 75 40 48 42 38 54 23 49
Singletons (%) 41(11.7) 35(14.1) 49(17.8) 33(26.8) 30(18.6) 17(9.6) 19(11.0) 34(14.3) 12(9.2) 34(13.6)
Doubletons (%) 7(2.0) 8(3.2) 9(3.3) 3(2.4) 8(5.0) 8(4.5) 9(5.2) 7(3.0) 5(3.8) 7(2.8)
Uniques (%) 19(33.3) 9(17.6) 18(24.3) 12(30.0) 12(25.5) 17(9.6) 4(9.5) 11(28.9) 15(27.8) 25(51.0)
Total ribotype richness (% ribotypes captured)
Chao1 33-100 32-95 48-100 41-79 45-93 33-67 30-66 38-90 18-42 30-92
ACE 8-100 18-91 40-100 38-71 39-84 27-55 26-55 24-87 12-36 13-86
ICE 8-100 18-91 40-100 38-71 39-84 27-55 26-55 24-87 12-36 36 13-86
Jack 1 33-48 35-49 51-68 45-59 49-64 35--48 32-45 41-55 19-28 32-46
Bacteria diversity
1-Simpson 0.49 0.56 0.81 0.94 0.95 0.88 0.89 0.89 0.76 0.51
Shannon 1.46 1.66 2.60 3.11 3.25 2.71 2.64 2.72 1.91 1.50

Estimators of ribotype richness (Chao1, ICE, ACE and Jack1) and their 95% confidence intervals were used to calculate the percentage range of total ribotypes captured in each sample.The Simpson index of diversity and Shannon index were used as a measure of the ribotype diversity. Rarefaction curves were used to interpolate total estimated richness and bacteria diversity for each of the FST samples to compare to the sample with the lowest number of sequenced clones (123 clones, sample 4). Note that sample 3 and 4 are from the same FST colony, but different storage conditions.