Estimators of ribotype richness (Chao1, ICE, ACE and Jack1) and their 95% confidence intervals were used to calculate the percentage range of total ribotypes captured in each sample.The Simpson index of diversity and Shannon index were used as a measure of the ribotype diversity. Rarefaction curves were used to interpolate total estimated richness and bacteria diversity for each of the FST samples to compare to the sample with the lowest number of sequenced clones (123 clones, sample 4). Note that sample 3 and 4 are from the same FST colony, but different storage conditions.