Table 4: Summary of Purification Steps of Alkaline Protease from Pseudomonas thermaerum

Purification Step Total Protein(mg) Total Enzyme Activity(U) Specific Activity(U/mg) % Recovery Purification Fold
Culture supernatant 117.4 2645 22.6 100 1
(NH)2SO Precipitation, dialyzed (0-60%) 36.70 1940.40 52.87 73.3 2.33
DEAE cellulose 1.79 246.15 137.54 9.3 6.08