Purification Step | Total Protein(mg) | Total Enzyme Activity(U) | Specific Activity(U/mg) | % Recovery | Purification Fold |
---|---|---|---|---|---|
Culture supernatant | 117.4 | 2645 | 22.6 | 100 | 1 |
(NHᶺ)2SOᶺ Precipitation, dialyzed (0-60%) | 36.70 | 1940.40 | 52.87 | 73.3 | 2.33 |
DEAE cellulose | 1.79 | 246.15 | 137.54 | 9.3 | 6.08 |