Fig. (4) Altered peroxisome proliferation in cells affected in AIM45 and YGR207c/CIR1 upon heat stress and antimycin A treatment, respectively. Mutant cells affected in AIM45 (aim45), YGR207c/CIR1 (cir1) and YOR356w/CIR2 (cir2) and the correspondent parental strain (BY4741), harboring a plasmid containing the green fluorescent protein gene fused with a peroxisome-targeting signal peptide (skl), were grown overnight on YPD, ressuspended in fresh medium, and incubated at 39ºC for 6h (A) or incubated in the presence of antimycin A at 30ºC for 28h (B) and cells containing more than 2 peroxisomes were counted on a fluorescence microscope. C: bright field and GFP fluorescence representative micrographs of strains BY4741 and ygr207c/cir1 from the antimycin A incubation experiment. *P < 0.05 when compared with strain BY4741.