Fig. (1) Multiplex PCR amplification of 165bp, 365bp and 541bp regions of the clinical specimens of M.tuberculosis and Nontubercular mycobacterioses on 2.5% agarose gel. Lane 1: 100bp Molecular Marker; Lane 2-12: Clinical specimens (2,4,6: Atypical); (3,5,7,9,10: M.Tuberculosis); (8: M.Bovis); (11,12: Inconclusive) (Lane 13: Positive Control; Lane 14: Negative Control.