Table 1.: Primers used in PCR Assays. Their Descriptions, Functions, Annealing Temperatures, Expected Amplicon Fragment Sizes and References

Gene	Function	Annealing Temp. (°C)	Amplicon Size (bp)	References
yjaA	Unknown function gene	55	211	[2]
tspE4.C2	DNA fragment	55	152	[2]
iucA	Aerobactin synthetase	55	1100	[21]
irp-2	Iron- controlling gene associated to yersiniabactin	59	287	[22]
fep C	Iron transport enterobactin	55	347	[23]
sitA	Iron transport	59	608	[25]
fyuA	Yersiniabactin	63	787	[24]
fimA	Structural subunit of type 1 fimbria	50	331	[25]
papA	Structural protein of P fimbria	65	328	[26]
crl	Regulatory gene of “curli” fimbria	54	250	[27]
csgA	Structural protein of “curli” fimbria	55	200	[27]
tsh	Temperature-sensitive hemagglutinin	61	420	[27]
lpfAO141	LPF fimbria	55	412	[28]
lpfAO154	LPF fimbria	55	525	[29]
iha	Adhesion protein	55	602	[30]