Table 1.: Primers used in PCR Assays. Their Descriptions, Functions, Annealing Temperatures, Expected Amplicon Fragment Sizes and References

Gene Function Annealing Temp. (°C) Amplicon Size (bp) References
yjaA Unknown function gene 55 211 [2]
tspE4.C2 DNA fragment 55 152 [2]
iucA Aerobactin synthetase 55 1100 [21]
irp-2 Iron- controlling gene associated to yersiniabactin 59 287 [22]
fep C Iron transport enterobactin 55 347 [23]
sitA Iron transport 59 608 [25]
fyuA Yersiniabactin 63 787 [24]
fimA Structural subunit of type 1 fimbria 50 331 [25]
papA Structural protein of P fimbria 65 328 [26]
crl Regulatory gene of “curli” fimbria 54 250 [27]
csgA Structural protein of “curli” fimbria 55 200 [27]
tsh Temperature-sensitive hemagglutinin 61 420 [27]
lpfAO141 LPF fimbria 55 412 [28]
lpfAO154 LPF fimbria 55 525 [29]
iha Adhesion protein 55 602 [30]

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